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The large quantity of acids produced causes a significant decrease in the pH of the culture medium. MRVP broth pH 6.
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The development of a stable red color on the surface of the medium indicates sufficient acid production to lower the pH to 4.
Because other organisms may produce smaller quantities of acid from the test substrate, an intermediate orange color between yellow and red may develop.
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Green married Martha Morrell and they had a son George Green, who had made 83 ascents with the Nassau balloon  After living in retirement for many years he died suddenly of heart disease at his residence, Ariel Villa, 51 Tufnell Park, Holloway, London, 26 March His monument is a small pedestal surmounted by the sculpted top half of a hot-air balloon.
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