Filter Sock 25um Micron Mesh Polypropylene

25micron~25um, 105mm×380mm (4.13inch x 14.96inch) polypropylene filter that removes everything larger than 0.025millimeters or 0.00098 of an inch!

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Filter Sock 25um Micron Mesh Polypropylene

This 105mm×380mm (4.13inch x 14.96inch) polypropylene is a really great tool to have if you are breeding any small aquatic creatures.
It filters out everything larger than 25micron which is an amazingly small 0.025millimeters or 0.00098 of an inch!
The two reasons you may need this is contamination, and collection of new species from wild cultures.

If you have open top tanks, or use your hand or tools like scoop nets without disinfecting them, then cross-contamination with new species is just a matter of time.
You may be careful as, but a small insect landing in you tank is all you need to introduce new species to your culture, especially if that insect was just outside.

In open top tanks up here where I am it’s bloody frogs!
I have to keep everything tightly lidded outside, and I just have to accept the cute little buggers and going to jump or squeeze in from one tank to the next mixing up the species constantly.
For this reason all the cultures I grow as individual species are in my contained sealed culture room, and each batch has multiple back-ups.
Each batch is sealed and closed to all outside contamination and regardless I constantly batch test my cultures.
My outside all start “pure” but as I grow so many, after a few seasons they are just mixes of several cool species.
It is never an issue the way I do things as the fish and poultry that eat them don’t mind at all.

In the wild it is flooding rains or birds drinking or feeding that normally causes the introduction of new species. Cladocera eggs that are trapped in the mud get stuck to the feet of birds and are carried from one pond to the next.

Enough of that, you get the idea.
If you only have a small culture of a species, and then you realize that another species you don’t want has been introduced, or you have several cultures but they end up mixed and you want to keep them separate for increased productivity, then the easiest things to do is dump the lot and come buy more pure live cultures from me.(I personally recommend that last option!).

The tricky thing to do is salvage the species, and start new cultures.
That’s easy too, just a little fiddly.
These filter socks make it a lot less of a gamble but I will get to that later.

What you traditionally want to do is remove one large healthy female with eggs, remove the water completely and wash her, then add that female to a new container of clean rainwater.
Firmly crossing your fingers she survives the stress of water chemistry change, and starts breeding happily again.
Unfortunately that very rarely happens..

To increase your odds you can just start a heap of cultures, each with just one healthy female with eggs.
Then if some die you just dump them, and if some are contaminated still just dump them too.
Keep only the very small % that are happily replicating and not contaminated.
Provided you start enough batches you should have success.

At one stage I had more than 500 small individual batches on the benches in constant rotation for just this reason.
Total nightmare to manage, but its the only way I knew how to get pure species cultures.
It does work, and I still do it that way with all my new species as though the sucess rate is very low the contamination rate is zero, which is very important in my situatio.
I then repeat that exact process again, and again, and again, for a dozen of so generations so I know with 10000% certainty there is no mixed species.
Unfortunately the shock of water chemistry change means that the survival percentage is always around 5% maximum with each batch, most often 0%, meaning I have to have a heap of containers, and a heap of aeration, very extensive paperwork and record keeping, and generally lots of stuffing around.

This new method I have developed is much simpler, and this is what I suggest you do if you want to select a species from wild collected eggs, or want to remove an unwanted species starting a new “pure” species culture on a small scale.
If you are a keen hobbiest, but don’t need lab like conditions and are just doing it for fun, then do this it works great!

Select several new clean culture containers or tanks.

Select several big fat healthy females with eggs and put them aside in a little of the original culture water.

Very carefully and slowly siphon the remaining culture water through the filter sock into the new containers, making sure that the culture water NEVER touches anywhere except deep the inside of the sock.

If your hands get wet with culture water, or you splash any in unfiltered, even as mist, or you do anything that may risk contamination, then immediately STOP.
Put everything down, wash, scrub, and/or disinfect your hands and all equipment involved, and start all over again.
Slow and steady folks, it saves a lot of time later.

The water that slowly flows through the 25 micron mesh comes out filtered and will have no particles big enough to cause issues with you new culture(at least in theory).

All the live animals and eggs, most of the algae and bacteria, and just about everything else will get stuck in the mesh of the sock, leaving only water and some pigments or dyes to come through.
Note that the water flowing through the mesh will not be crystal clear due to the dye effect algae pigments have on the water, but it will not be chunky or full of life.

This filtered water will be very very similar chemically to the original culture the mature females are used to, so they are very likely to survive the change.

Remove your individual healthy females with eggs from the original culture water.

Wash them, remove all water, then add only ONE of them to each newly filtered batch.
Make sure NONE of the original culture water has entered with them or all your work will be for nothing.

In theory, you now have “clean” water, that the creature is already adapted to, so she is much more likely to survive and thrive in it.
25%-50% success is about what I get using this method, so as long as you split 1 culture into 4 or more new cultures, you should have at least one winner.

Using this method I still have some females die from the shock and stress of it all, and I still have some batches contaminated by hitch-hiking of other species, but on the whole, generally speaking, it works great.

It is at least 20x easier and faster that the original much more time consuming but much more exact method, and for backyard hobbiest, this is what I suggest you do.